Part:BBa_K797005:Experience

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Applications of BBa_K797005

BBa_K797005 was transformed into BL21(DE3). Cells were precultured overnight and diluted into fresh SOC medium. IPTG was added when OD600 was approx. 0.7, then cells were incubated for 2h at 37°C. Total RNA was extracted and reverse transcripted.
cDNA was used as templates of RT-PCR, shown in the following figure.

Fig.3-6 RT-PCR of R9:GFP
Lane1:100bp ladder
Lane2:GAPDH (Negative control)
Lane3:GAPDH(R9-GFP)
Lane4:GFP (Negative control)
Lane5:GFP (R9-GFP)

This result indicates mRNA of R9-GFP is expressed.

User Reviews

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